Volume 5, Issue 1, January 2017, Page: 21-26
Evaluation of Seasonal Serum Level of Estradiol and Progesterone Hormones in Iranian Female Caucasian Viper
Saeedeh Fani, Basic Sciences Faculty, Islamic Azad University, Damqan, Iran
Qulam Hussein Vaezi, Basic Sciences Faculty, Islamic Azad University, Damqan, Iran
Fatemeh Todehdehghan, Venomous Animals Department, Razi Vaccine & Serum Research institute, Karaj, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
Received: Oct. 23, 2016;       Accepted: Feb. 13, 2017;       Published: Mar. 4, 2017
DOI: 10.11648/j.avs.20170501.14      View  1898      Downloads  77
Abstract
Steroids hormones have a basic role in animals' reproductive activity and their secretion amounts are changed during the year with animal physiological conditions. There is no study reporting Iranian female snake's hormones controlling reproductive activity. In this study the estradiol (E2) and progesterone (P4) hormones level in serum samples of 26 female Caucasian vipers were examined by electrochemiluminescence (ECL). The results show the average concentration of estradiol (E2) hormone in the autumn (157.2±47.53 nmol/ Lt) is less than the early winter (December-January), 318.33±156nmol/l (p≤0.1) and again the concentration of this hormone reduced in the summer, 188.94±33.57 nmol/Lt (p≤0.78). The average concentration of P4 hormone in the autumn was 0.45±0.14nmol/Lt and in winter was 0.96±0.57 nmol/Lt, that show increasing process (p≤0.22). The average concentration of this hormone is decreasing in the summer, 0.82±0.23 nmol/Lt as compared to the winter (p≤0.78). In the fertile snacks the amount of serums estradiol is increased during the vitellogenesis and progesterone is increased during pregnancy and the amount of these hormones is low throughout the year in unfertile snacks. According to these hormones changing during the seasons it seems that the Iranian Caucasian viper from Lar area in Tehran province, during the winter season is in the vitellogenesis phase and summer is the pregnancy time and the autumn is the time of postpartum. Although the maximum amount of progesterone is inconsistent with the winter but like this case is reported before.
Keywords
Female Viper, Steroid Hormone, Season Changes, Serum, Electrochemiluminescence
To cite this article
Saeedeh Fani, Qulam Hussein Vaezi, Fatemeh Todehdehghan, Evaluation of Seasonal Serum Level of Estradiol and Progesterone Hormones in Iranian Female Caucasian Viper, Animal and Veterinary Sciences. Vol. 5, No. 1, 2017, pp. 21-26. doi: 10.11648/j.avs.20170501.14
Copyright
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Reference
[1]
Afsharzadeh N. Shiravi A. H. and Todehdehghan F. 2015. Seasonal changes in ovarian follicle growth in Iran viper (Vipera albicornuta.) Advances in Biology, 2015, 9-19.
[2]
Aldridge R. D. and Arackal, A. A. 2005. Reproductive biology and stressof captivity in male brown treesnakes (Boiga irregularis) on Guam. Aust. J. Zool. 53, 249-256.
[3]
Aldridge R. D., Duvall D., 2002. Evolution of the mating season in the pitvipers of North America. Herpetol. Monographs 16, 1–25.
[4]
Almeida-Santos S. M., Abdalla F. M. F., Silveira P. F., Breno, Y. N. and Saloma ˜o, M. G. 2004a. Reproductive cycle of the Neotropical Crotalus durissus terrificus: I. Seasonal levels and interplay between steroid hormones and vasotocinase. Gen. Comp. Endocrinol., 139, 143-150.
[5]
Almeida-Santos S. M., Laporta-Ferreira L. L., Antoniazzi M. M., Jared C. 2004b. Sperm storage in males of the snake Crotalus durissus terrificus (Crotalinae: Viperidae) in southeastern Brazil. Comp. Biochem. Phys. 139, 169–174.
[6]
Bonnet X., Naulleau G., Bradshaw D., Shine R. 2001. Changes in plasma progesterone in relation to vitellogenesis and gestation in the viviparous snake Vipera aspis. Gen. Comp. Endocrinol. 121, 84–94.
[7]
Callard I. P., Hirsch M., 1976. The influence of oestradiol-17β and progesterone on the contractility of the oviduct of the turtle, Chrysemys picta, in vitro. J. Endocrinol. 68, 147–152.
[8]
Callard I. P., Riley D., Perez L. 1990. Vertebrate vitellogenesis: molecular model for multi hormonal control of gene regulation. Prog. Clin. Biol. Res. 342, 343–348.
[9]
Graham, S. P., Earley, R. L., Hoss, S. K., Schuett, G. W., 2008. The reproductive biology of male cottonmouths (Agkistrodon piscivorus): Do plasma steroid hormones predict the mating season? Gen. Comp. Endocrinol. 159, 226-235.
[10]
Katsu Y., Matsubara, K., Kohno S., Matsuda Y., Toriba M., Oka K., Louis J. Guillette, J. R.., Ohta Y., and Iguchi T. 2010. Molecular cloning, characterization, and chromosome mapping of reptilian estrogen receptors. Endocrinology, 151 (12): 5710–5720.
[11]
Latifi M. 2000. Iran snakes, environment maintenance organization, pp. 10-461.
[12]
Lind C. M., Husak J. F., Eikenaar C., Moore I. T., Taylor E. N. 2010. The relationship between plasma steroid hormone concentrations and the reproductive cycle in the Northern Pacific rattlesnake, Crotalus oreganus, Gen. Comp. Endocrinol.166, 590–599.
[13]
Ramsay J. M., WatralV., SchreckC. B., Kent M. L. 2009. Husbandry stress exacerbates mycobacterial infections in adult zebrafish, Danio rerio (Hamilton). 10, 1365-2761.
[14]
Saint Girons H., Bradshaw S. D., Bradshaw F. J. 1993. Sexual activity and plasma levels of sex steroids in the aspic viper Vipera aspis L. (Reptilia, Viperidae). Gen Comp Endocrinol 91: 287–297.
[15]
Schuett, G. W., 1992. Is long-term sperm storage an important component of the reproductive biology of temperate pitvipers? In: Campbell, J. A., Brodie, E. D., Jr. (Eds.), Biology of the Pitvipers. Selva, Tyler, Texas, pp. 169–184.
[16]
Schuett G. W., Harlow H. J., Rose J. D., Van Kirk E. A., Murdoch W. J., 1997. Annualcycle of testosterone in male copperheads, Agkistrodon contortix (Serpentes: Viperidae): relationship to timing of spermatogenesis, mating, and agonistic behavior. Gen. Comp. Endocrinol. 105, 417–424.
[17]
Schuett G. W., Carlisle S. L., Holycross A. T., OLeile J. K., Hardy Sr D. L., Van Kirk E. A., Murdoch W. J., 2002. Mating system of male Mojave Rattlesnakes (Crotalusscutulatus): seasonal timing of mating, agonistic behavior, spermatogenesis, sexualsegment of the kidney, and plasma sex steroids. In: Schuett, G. W., Hoggren, M., Douglas, M. E., Greene, H. W. (Eds.), Biology of the Vipers. Eagle Mountain Publishing, Eagle Mountain, Utah, pp. 515–532.
[18]
Schuett G. W., Hardy Sr D. L., Greene H. W., Earley R. L., Grober M. S., Van KirkE. A., Murdoch W. J., 2005. Sympatric rattlesnakes with contrasting mating systems show differences in seasonal patterns of plasma sex steroids. Anim. Behav. 70, 257–266.
[19]
Schuett, G. W., Repp, R. A., Taylor, E. N., DeNardo, D. F., Earley, R. L., Van Kirk, E. A., Murdoch, W. J., 2006. Winter profile of plasma sex steroid levels in free-living male western diamond-backed rattlesnakes, Crotalus atrox (Serpentes: Viperidae). Gen. Comp. Endocrinol. 149, 72–80.
[20]
ShakooriS., Todehdehghan F., Shiravi A. H., Hojati V. 2015. The assessment of captive breeding in the Caucasian viper (Gloydius halys caucasicus) in Iran. Journal of Entomology and Zoology Studies 3 (2): 257-259.
[21]
Shannon K., Hoss A., Mark J. Garcia B., Ryan L., Earley B., Rulon W., Clark A. 2014. Fine-scale hormonal patterns associated with birth and maternal care in the cottonmouth (Agkistrodon piscivorus), a North American pit viper snake. Gen. Comp. Endocrinol. 208, 85-93.
[22]
SiegelD. S., Sever D. M., Rheubert J. L., Gribbins K. M. 2009. Reproductive biology of Agkistrodon piscivorus Lacépède (Squamata, Serpents, Viperidae, Crotalinae). Herpetol. Monogr. 23, 74–107.
[23]
Smith C. F., Schuett G. W., and Hoss S. K. 2012. Reproduction in female copperhead snakes (Agkistrodon contortrix): plasma steroid profiles during gestation and post-birth periods. Zoological Science 29 (4): 273–279.
[24]
Taylor EN, DeNardo D. F. 2011. Hormone and reproductive cycles in snakes. In “Hormones and Reproduction in Vertebrates, Vol. 3, Reptiles” Ed by DO Norris, KH Lopez, Acade mic Press, San Diego, California, pp 355–372.
[25]
Taylor E. N., DeNardo, D. F., 2010. Hormones and reproductive cycles of snakes, In"Hormones and Reproduction of Vertebrates, (D. O. Norris and K. H. Lopez,eds.), San Diego, Elsevier.
[26]
Taylor, E. N., DeNardo, D. F., Jennings, D. H. 2004. Seasonal steroid hormone levels and their relation to reproduction in the western diamond-backed rattlesnake, Crotalus atrox (Serpentes: Viperidae). Gen. Comp. Endocrinol.136, 328–337.
[27]
Todehdehqan. F. 2014. Tavalod Marha, Frst edition, Avaye Fahim publication, Tehran, Iran.
[28]
Tsai TS, MC Tu. 2001. Reproductive cycle of female Chinese green tree viper, Trimeresurus stejnegeri stejnegeri in northern Taiwan. Herpetologica 57: 157-168.
[29]
Zaidan III, F., Kreider, D. L., Beaupre, S. J., 2003. Testosterone cycles and reproductive energetics: implications for northern range limits of the cottonmouth (Agkistrodon piscivorus leucostoma). Copeia 2003, 231–240.
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