Volume 4, Issue 6, November 2016, Page: 93-96
The Prevalence of Melissococcus plutonius by Polymerase Chain Reaction in Kurdistan apiaries, Iran
Mohammad Khezri, Veterinary Research Department, Agricultural and Natural Resources Research and Education Center, AREEO, Sanandaj, Iran
Mojtaba Moharami, Department of Honeybee, Silkworm and Wildlife Research Disease, Razi Vaccine and Serum Research Institute, AREEO, Karaj, Iran
Hussein Modirrousta, Department of Honeybee, Silkworm and Wildlife Research Disease, Razi Vaccine and Serum Research Institute, AREEO, Karaj, Iran
Zahra Salahi, Department of Honeybee, Silkworm and Wildlife Research Disease, Razi Vaccine and Serum Research Institute, AREEO, Karaj, Iran
Babak Rokhzad, Veterinary Research Department, Agricultural and Natural Resources Research and Education Center, AREEO, Sanandaj, Iran
Received: Jul. 12, 2016;       Accepted: Jul. 26, 2016;       Published: Dec. 14, 2016
DOI: 10.11648/j.avs.20160406.12      View  2610      Downloads  68
Abstract
European foulbrood (EFB) is caused by bacterial agents Melissococcus plutonius and is considered of major importance because it can be spread through many bee products including honey, which is internationally widely traded. A polymerase chain reaction (PCR) molecular diagnosis survey for honey bee pathogen EFB was conducted on European honey bee (EHB), Apis mellifera, population sampled from Kurdistan apiaries (n=100). For multiplex PCR amplification of partial 16S rRNA (= SSU rRNA) gene fragments (486 bp), was conducted on 100 samples. Results showed that of 100 samples only 4 samples (4%) were positive for M. plutonius in Kurdistan apiaries. This is the first study to document the occurrence of EFB by PCR assay in Kurdistan apiaries. It seems that surveys are required to characterize the distribution and prevalence of M. plutonius in different regions of Iran.
Keywords
M. plutonius, European foulbrood, PCR, Kurdistan apiaries
To cite this article
Mohammad Khezri, Mojtaba Moharami, Hussein Modirrousta, Zahra Salahi, Babak Rokhzad, The Prevalence of Melissococcus plutonius by Polymerase Chain Reaction in Kurdistan apiaries, Iran, Animal and Veterinary Sciences. Vol. 4, No. 6, 2016, pp. 93-96. doi: 10.11648/j.avs.20160406.12
Copyright
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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