Volume 4, Issue 1, January 2016, Page: 1-4
Investigation of Rotavirus Infection in Calves with Diarrhea in Northeast Turkey
Volkan Yilmaz, Department of Virology, Faculty of Veterinary Medicine, University of Kafkas, Kars, Turkey
Received: Dec. 8, 2015;       Accepted: Dec. 16, 2015;       Published: Dec. 30, 2015
DOI: 10.11648/j.avs.20160401.11      View  3517      Downloads  142
Abstract
Rotavirus is the most common cause of severe diarrhea in newborn calves. In this study, it was aimed to investigate frequency of Rotavirus infections in calves in Kars region in Northeast Turkey by feces sampling 112 diarrheic calves of 0-6 months old. Feces samples were tested for Rotavirus antigen by Enzyme Linked Immunosorbent Assay (ELISA). Of the 112 feces samples examined by ELISA 10 samples were identified as positive (8.92%). At the molecular stage, reverse transcription polymerase chain reaction (RT-PCR) technique was used to determine the presence of Rotavirus nucleic acid by using specific generic primers for VP6 genetic regions in feces samples. The 10 samples that were rotavirus antigen positive by ELISA were also positive on RT-PCR technique. The proportion of positivity in feces samples suggested that rotavirus infections are common in the Kars region in Northeast Turkey.
Keywords
Calves, Diarrhea, ELISA, RT-PCR
To cite this article
Volkan Yilmaz, Investigation of Rotavirus Infection in Calves with Diarrhea in Northeast Turkey, Animal and Veterinary Sciences. Vol. 4, No. 1, 2016, pp. 1-4. doi: 10.11648/j.avs.20160401.11
Copyright
Copyright © 2015 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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