Volume 7, Issue 2, June 2019, Page: 27-33
Genetic Diversity of a Collection of Solanum macrocarpon from Burkina Faso Revealed by Microsatellite Markers
Kabore Boukare, Training and Research Unit in Life and Earth Sciences, University Joseph KI-ZERBO, Ouagadougou, Burkina Faso
Sawadogo Boureima, Training and Research Unit in Life and Earth Sciences, University Joseph KI-ZERBO, Ouagadougou, Burkina Faso
Kiebre Mariam, Training and Research Unit in Life and Earth Sciences, University Joseph KI-ZERBO, Ouagadougou, Burkina Faso
Tiama Djakaridia, Training and Research Unit in Life and Earth Sciences, University Joseph KI-ZERBO, Ouagadougou, Burkina Faso
Kiebre Zakaria, Training and Research Unit in Life and Earth Sciences, University Joseph KI-ZERBO, Ouagadougou, Burkina Faso
Traore Renan Ernest, Training and Research Unit in Life and Earth Sciences, University Joseph KI-ZERBO, Ouagadougou, Burkina Faso
Sawadogo Mahamadou, Training and Research Unit in Life and Earth Sciences, University Joseph KI-ZERBO, Ouagadougou, Burkina Faso
Bationo-Kando Pauline, Training and Research Unit in Life and Earth Sciences, University Joseph KI-ZERBO, Ouagadougou, Burkina Faso
Received: Mar. 17, 2019;       Accepted: Jun. 13, 2019;       Published: Jun. 27, 2019
DOI: 10.11648/j.ijgg.20190702.12      View  179      Downloads  46
Abstract
Solanum macrocarpon is an important vegetable native to Africa, with leaves and fruits. It is a potential source of income and nutrients that contribute to the nutritional balance of the local population's food intake. However, S. macrocarpon did not attract the interest of researchers in relation to other Solanum species. Indeed, knowledge of the genetic diversity of S. macrocarpon (Gboma Eggplant) in Africa is limited. Although agronomic variations and relationships generally reveal important traits of interest to breeders. This study attempts to estimate the genetic diversity of a collection of S. macrocarpon (32 accessions) from Burkina Faso using SSR markers. The results show that eleven (11) of the 22 SSR markers tested were informative for phylogenetic analysis and could serve as a perfect reference for diversity studies. The 11 polymorphic SSR primers selected amplified 22 alleles with an average of 2 per primer. The polymorphism information content (PIC) varied from 0.212 for emg01B17 to 0.437 for emd15D09. The genetic distances between the accessions studied varied from 0 to 0.68 and made it possible to distinguish three genetic groups. The expected Nei (He) heterozygosity for the genetic group ranged from 0.140 for group III to 0.261 for group I. The eleven pairs of SSR markers sufficiently discriminated against the accessions of S. macrocarpon. The results of this study will be useful for the conservation and genetic improvement of the S. macrocarpon species in Burkina Faso.
Keywords
Genetic diversity, Polymorphism, S. macrocarpon, SSR Markers, Burkina Faso
To cite this article
Kabore Boukare, Sawadogo Boureima, Kiebre Mariam, Tiama Djakaridia, Kiebre Zakaria, Traore Renan Ernest, Sawadogo Mahamadou, Bationo-Kando Pauline, Genetic Diversity of a Collection of Solanum macrocarpon from Burkina Faso Revealed by Microsatellite Markers, International Journal of Genetics and Genomics. Vol. 7, No. 2, 2019, pp. 27-33. doi: 10.11648/j.ijgg.20190702.12
Copyright
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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