Volume 7, Issue 2, June 2019, Page: 18-26
X-ray Induced Genetic Variability in Amaranthus hybridus L. and Analysis of Variants Using Morphological and Random Amplified Polymorphic DNA Data
Beluchukwu Joseph Nwankwo, Department of Plant Science and Biotechnology, Michael Okpara University of Agriculture, Umudike, Nigeria
Garuba Omosun, Department of Plant Science and Biotechnology, Michael Okpara University of Agriculture, Umudike, Nigeria
Hilary Odo Edeoga, Department of Plant Science and Biotechnology, Michael Okpara University of Agriculture, Umudike, Nigeria
Odoligie Imarhiagbe, Department of Biological Sciences, Edo University, Iyamho, Nigeria
Jeffrey Iyare Omoruyi, Rubber Research Institute of Nigeria, Iyanomo, Nigeria
Edeji Franklin Uzodinma, Department of Biotechnology, Federal Institute of Industrial Research, Oshodi, Nigeria
Received: Apr. 5, 2019;       Accepted: May 28, 2019;       Published: Jun. 26, 2019
DOI: 10.11648/j.ijgg.20190702.11      View  172      Downloads  23
Abstract
Amaranthus hybridus L. is an important vegetable in Nigeria, grown mainly for its highly nutritious leaves. In a bid to explore the possibility of increasing the infra-specific diversity of the crop, we induced three accessions of A. hybridus from Anambra State, Nigeria with x-ray particles using various concentration levels. The experimental layout composed a 5 x 3 x 3 Randomized Complete Block Design arrangement, with treatment levels: 1 MGY, 2 MGY, 4 MGY, and 6 MGY, selectively allotted to the different accession based on a preliminary dosimetry test. Morphological data were recorded and subsequently leaves samples subjected to Deoxyribonucleic Acid (DNA) extraction and Random Amplified Polymorphic DNA (RAPD) analysis. The results show that except for inflorescence weight, other morphological data were not statistically significant. However, RAPD techniques identify some variations induced by x-ray particles on the different accessions. Ogbunike accession irradiated with 4 MGY appears to show the optimum concentration of x-ray particles required to induce genetic variability in A. hybridus. These findings are of paramount importance to plant breeders, whose primary tool is to identify variations to produce better yielding varieties.
Keywords
X-Ray, Amaranthus hybridus L., Genetic Variability, Mutation, Diversity, RAPD
To cite this article
Beluchukwu Joseph Nwankwo, Garuba Omosun, Hilary Odo Edeoga, Odoligie Imarhiagbe, Jeffrey Iyare Omoruyi, Edeji Franklin Uzodinma, X-ray Induced Genetic Variability in Amaranthus hybridus L. and Analysis of Variants Using Morphological and Random Amplified Polymorphic DNA Data, International Journal of Genetics and Genomics. Vol. 7, No. 2, 2019, pp. 18-26. doi: 10.11648/j.ijgg.20190702.11
Copyright
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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