Volume 6, Issue 1, March 2018, Page: 8-10
3D Diffraction – Limited Imaging with a Laser Fourier Holographic Microscope
Vladimir Boris Karpov, Coherent and Nonlinear Optics Department, A. M. Prokhorov General Physics Institute, Russian Academy of Sciences, Moscow, Russia
Received: Dec. 6, 2016;       Accepted: Aug. 17, 2017;       Published: Mar. 26, 2018
DOI: 10.11648/j.ijgg.20180601.12      View  1332      Downloads  71
Abstract
A laser holographic microscope (LHM) is investigated experimentally. The standard slide of Parascaris Univalens Iarva (ascaris) is studied. Comparison of the pictures of the same ascaris cell, observed by the LHM and high-performance Nikon conventional optical microscope (COM) 10×100/1.25 with immersion oil and green filter indicates that the both microscopes provide diffraction – limited 3-D spatial resolution, but dramatically different contrast. Thus, the LHM gives much more subcellular information.
Keywords
Speckle-Noise, Fourier Holography, Mach – Zehnder Scheme, CCD Detector, Digital Image Reconstruction
To cite this article
Vladimir Boris Karpov, 3D Diffraction – Limited Imaging with a Laser Fourier Holographic Microscope, International Journal of Genetics and Genomics. Vol. 6, No. 1, 2018, pp. 8-10. doi: 10.11648/j.ijgg.20180601.12
Copyright
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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