Volume 6, Issue 1, March 2018, Page: 8-10
3D Diffraction – Limited Imaging with a Laser Fourier Holographic Microscope
Vladimir Boris Karpov, Coherent and Nonlinear Optics Department, A. M. Prokhorov General Physics Institute, Russian Academy of Sciences, Moscow, Russia
Received: Dec. 6, 2016;       Accepted: Aug. 17, 2017;       Published: Mar. 26, 2018
DOI: 10.11648/j.ijgg.20180601.12      View  2138      Downloads  132
A laser holographic microscope (LHM) is investigated experimentally. The standard slide of Parascaris Univalens Iarva (ascaris) is studied. Comparison of the pictures of the same ascaris cell, observed by the LHM and high-performance Nikon conventional optical microscope (COM) 10×100/1.25 with immersion oil and green filter indicates that the both microscopes provide diffraction – limited 3-D spatial resolution, but dramatically different contrast. Thus, the LHM gives much more subcellular information.
Speckle-Noise, Fourier Holography, Mach – Zehnder Scheme, CCD Detector, Digital Image Reconstruction
To cite this article
Vladimir Boris Karpov, 3D Diffraction – Limited Imaging with a Laser Fourier Holographic Microscope, International Journal of Genetics and Genomics. Vol. 6, No. 1, 2018, pp. 8-10. doi: 10.11648/j.ijgg.20180601.12
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