Volume 1, Issue 2, June 2013, Page: 13-21
Optimization, Characterization and In Vitro Evaluation of Entomopathogenic Fungal Exopolysaccharides as Prebiotic
Wai Prathumpai, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, 113 Thailand Science Park, Klong Nueng, Klong Luang, Pathum Thani 12120, Thailand
Pranee Rachathewee, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, 113 Thailand Science Park, Klong Nueng, Klong Luang, Pathum Thani 12120, Thailand
Sutamat Khajeeram, Department of Biotechnology, Thammasat University, Klong Nueng, Klong Luang, Pathumthani 12120, Thailand
Jean-Jacques Sanglier, Novartis Institute for BioMedical Research Basel, Natural Products Unit, Novartis Pharma AG, Basel, Switzerland
Pariyada Tanjak, National Metal and Materials Technology Center (MTEC), 114 Thailand Science Park, Paholyothin Rd., Klong Nueng, Klong Luang, Pathum Thani 12120, Thailand
Pawadee Methacanon, National Metal and Materials Technology Center (MTEC), 114 Thailand Science Park, Paholyothin Rd., Klong Nueng, Klong Luang, Pathum Thani 12120, Thailand
Received: Mar. 5, 2013;       Published: Jun. 20, 2013
DOI: 10.11648/j.ab.20130102.12      View  2822      Downloads  156
Optimization of exopolysaccharides (EPS) produced by three strains of entomopathogenic fungi (Beauveria bassiana BCC 2692, Ophiocordyceps dipterigena BCC 2073, and Paecilomyces tenuipes BCC 2656) was carried out together with analyses of their prebiotic properties. B. bassiana BCC 2692 produced 6.27±0.22 g/L EPS on optimal medium using two-level fractional factorial design and 4.7 g/L EPS in bioreactor. EPS productions of O. dipterigena BCC 2073 were 13.2 g/L and 41.2 g/L in shake flask and bioreactor, respectively. For P. tenuipes BCC 2656, 1.47±0.21 g/L EPS in shake flask and 28.1 g/L EPS in bioreactor were obtained. These EPS were previously characterized as -glucan with differences in molecular weights and degree of branching. They were resistant to hydrolysis by both hydrochloric acid and porcine pancreatic α-amylase. Furthermore, when used as the sole carbon source, all three types of EPS supported growth in vitro of two different probiotic bacteria (Lactobacillus acidoplilus BCC 13839 and Bifidobacterium animalis ATCC 25527). A constant viability of L. acidophilus BCC 13839 was maintained throughout the cultivation period (48 hours) on all three entomopatogenic fungal EPS. All EPS also supported better growth and maintained longer growth period of B. animalis ATCC 25527 than glucose or inulin. Thus these entomopathogenic fungi EPS are promising candidates in prebiotic industry, expanding the pool of current commercial prebiotics.
Exopolysaccharide, Glucan, Prebiotic, Probiotic, Entomopathogenic Fungi
To cite this article
Wai Prathumpai, Pranee Rachathewee, Sutamat Khajeeram, Jean-Jacques Sanglier, Pariyada Tanjak, Pawadee Methacanon, Optimization, Characterization and In Vitro Evaluation of Entomopathogenic Fungal Exopolysaccharides as Prebiotic, Advances in Biochemistry. Vol. 1, No. 2, 2013, pp. 13-21. doi: 10.11648/j.ab.20130102.12
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