Volume 1, Issue 2, June 2013, Page: 13-21
Optimization, Characterization and In Vitro Evaluation of Entomopathogenic Fungal Exopolysaccharides as Prebiotic
Wai Prathumpai, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, 113 Thailand Science Park, Klong Nueng, Klong Luang, Pathum Thani 12120, Thailand
Pranee Rachathewee, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, 113 Thailand Science Park, Klong Nueng, Klong Luang, Pathum Thani 12120, Thailand
Sutamat Khajeeram, Department of Biotechnology, Thammasat University, Klong Nueng, Klong Luang, Pathumthani 12120, Thailand
Jean-Jacques Sanglier, Novartis Institute for BioMedical Research Basel, Natural Products Unit, Novartis Pharma AG, Basel, Switzerland
Pariyada Tanjak, National Metal and Materials Technology Center (MTEC), 114 Thailand Science Park, Paholyothin Rd., Klong Nueng, Klong Luang, Pathum Thani 12120, Thailand
Pawadee Methacanon, National Metal and Materials Technology Center (MTEC), 114 Thailand Science Park, Paholyothin Rd., Klong Nueng, Klong Luang, Pathum Thani 12120, Thailand
Received: Mar. 5, 2013;       Published: Jun. 20, 2013
DOI: 10.11648/j.ab.20130102.12      View  2822      Downloads  156
Abstract
Optimization of exopolysaccharides (EPS) produced by three strains of entomopathogenic fungi (Beauveria bassiana BCC 2692, Ophiocordyceps dipterigena BCC 2073, and Paecilomyces tenuipes BCC 2656) was carried out together with analyses of their prebiotic properties. B. bassiana BCC 2692 produced 6.27±0.22 g/L EPS on optimal medium using two-level fractional factorial design and 4.7 g/L EPS in bioreactor. EPS productions of O. dipterigena BCC 2073 were 13.2 g/L and 41.2 g/L in shake flask and bioreactor, respectively. For P. tenuipes BCC 2656, 1.47±0.21 g/L EPS in shake flask and 28.1 g/L EPS in bioreactor were obtained. These EPS were previously characterized as -glucan with differences in molecular weights and degree of branching. They were resistant to hydrolysis by both hydrochloric acid and porcine pancreatic α-amylase. Furthermore, when used as the sole carbon source, all three types of EPS supported growth in vitro of two different probiotic bacteria (Lactobacillus acidoplilus BCC 13839 and Bifidobacterium animalis ATCC 25527). A constant viability of L. acidophilus BCC 13839 was maintained throughout the cultivation period (48 hours) on all three entomopatogenic fungal EPS. All EPS also supported better growth and maintained longer growth period of B. animalis ATCC 25527 than glucose or inulin. Thus these entomopathogenic fungi EPS are promising candidates in prebiotic industry, expanding the pool of current commercial prebiotics.
Keywords
Exopolysaccharide, Glucan, Prebiotic, Probiotic, Entomopathogenic Fungi
To cite this article
Wai Prathumpai, Pranee Rachathewee, Sutamat Khajeeram, Jean-Jacques Sanglier, Pariyada Tanjak, Pawadee Methacanon, Optimization, Characterization and In Vitro Evaluation of Entomopathogenic Fungal Exopolysaccharides as Prebiotic, Advances in Biochemistry. Vol. 1, No. 2, 2013, pp. 13-21. doi: 10.11648/j.ab.20130102.12
Reference
[1]
O. Bañuelos, L. Fernández, J.M. Corral, M. Valdivieso-Ugarte, J.L. Adrio, and J. Velasco, "Metabolism of prebiotic products containing b (2-1) fructan mixtures by two Lactobacillus strains," Anaerobe, vol. 14, pp.:184-189, June 2008.
[2]
D. Bosscher, J. Van Loo, and A. Franck, "Inulin and oligofructose as prebiotics in the prevention of intestinal infections and diseases," Nutr. Res. Rev., vol. 19, pp. 216-226, December 2006.
[3]
E. Bruzzese, M. Volpicelli, V. Squeglia, D. Bruzzese, F. Salvini, M. Bisceglia, P. Lionetti, M. Cinquetti, G. Iacono, S. Amarri, and A. Guarino, "A formula containing galacto- and fructo-oligosaccharides prevents intestinal and extra-intestinal infections: an observational study," Clin. Nutr., vol. 28, pp. 156-161, April 2009.
[4]
M.F. Chaplin, "Monosaccharides," in Carbohydrate analysis: a practical approach, M.F. Chaplin and J.F. Kennedy, Eds. Oxford: IRL Press, 1986, pp. 1-36.
[5]
W. Chen, Z. Zhao, S. F. Chen, and Y. Q. Li, "Optimization for the production of exopolysaccharide from Fomes fomentarius in submerged culture and its antitumor effect in vitro," Bioresour. Technol., vol. 99, pp. 3187-3194, May 2008.
[6]
G.V. Coppa, L. Zampini, T. Galeazzi, and O. Gabrielli, "Prebiotics in human milk: a review," Dig. Liver Dis., vol. 38, pp. S291-S294, December 2006.
[7]
J.H. Cummings, G.T. Macfarlane, and H.N. Englyst, "Prebiotic digestion and fermentation," Am. J. Clin. Nutr., vol. 73, pp. 415S-420S, February 2001.
[8]
L.C. Douglas and M.E. Sanders, "Probiotics and prebiotics in dietetics practice," Am. J. Clin. Nutr., vol. 108, pp. 510-521, March 2008.
[9]
Falony, G., A. Verschaeren, F. De Bruycker, V. De Preter, K. Verbeke, F. Leroy, and L. De Vuyst, "In vitro kinetics of prebiotic inulin-type fructan fermentation by butyrate-producing colon bacteria: implementation of online gas chromatography for quantitative analysis of carbon dioxide and hydrogen gas production," Appl. Environ. Microbiol., vol. 75, pp. 5884-5892, September 2009.
[10]
G. Falony, K. Lazidou, A. Verschaeren, S. Weckx, D. Maes, and L. De Vuyst, "In vitro kinetic analysis of fermentation of prebiotic inulin-type fructans by Bifidobacterium species reveals four different phenotypes," Appl. Environ. Microbiol., vol. 75, pp. 454-461, September 2009.
[11]
K. Ghosh, K. Chandra, A.K. Ojha, and S.S. Islam, "NMR and MALDI-TOF analysis of a water-soluble glucan from an edible mushroom Volvariella diplasia," Carbohydr. Res., vol. 343, pp. 2834-2840, November 2008.
[12]
J.T.M. Hamilton-Miller, "Probiotics and prebiotics in the elderly," Postgrad. Med. J., vol. 80, pp. 447-451, January 2004.
[13]
D.C. Hernot, T.W. Boileau, L.L. Bauer, I.S. Middelbos, M.R. Murphy, K.S. Swanson, and G.C.Jr. Fahey, "In vitro fermentation profiles, gas production rates, and microbiota modulation as affected by certain fructans, galactooligosaccharides, and polydextrose," J. Agric. Food. Chem., vol. 57, pp. 1354-1361, February 2009.
[14]
S.J. Jung, R. Houde, B. Baurhoo, X. Zhao, and B.H. Lee, "Effects of galacto-oligosaccharides and a Bifidobacteria lactis-based probiotic strain on the growth performance and fecal microflora of broiler chickens," Poult. Sci., vol. 87, pp. 1694-1699, September 2008.
[15]
G. Kelly, "Inulin-type prebiotics-a review: part 1," Altern. Med. Rev., vol. 13, pp. 315-29, December 2008.
[16]
G. Kelly, "Inulin-type prebiotics: a review (Part 2)," Altern. Med. Rev., vol. 14, pp. 36-55, March 2009.
[17]
Y.T. Kim, E.H. Kim, C. Cheong, D.L. Williams, C.W. Kim, and S.T. Lim, "Structural characterization of -D-(1-3, 1-6)-linked glucans using NMR spectroscopy," Carbohydr. Res., vol. 328, pp. 331-341, September 2000.
[18]
M. Kiyohara, A. Tachizawa, M. Nishimoto, M. Kitaoka, H. Ashida, and K. Yamamoto, "Prebiotic effect of lacto-N-biose I on bifidobacterial growth," Biosci. Biotechnol. Biochem., vol. 73, pp. 1175-1179, May 2009.
[19]
K. Kocharin, P. Rachathewee, J.J. Sanglier, and W. Prathumpai, "EBP production of Ophiocordyceps dipterigena BCC 2073: optimization, production in bioreactor and characterization," BMC Biotechnol. vol .12, pp. 10:51, July 2010.
[20]
S. Kolida and G.R. Gibson, "Prebiotic capacity of inulin-type fructans," J. Nutr., vol. 137, pp. 2503S-2506S, November 2007.
[21]
D. Li, J.M. Kim, Z. Jin, and J. Zhou, "Prebiotic effectiveness of inulin extracted from edible burdock," Anaerobe., Vol. 14, pp. 29-34, February 2008.
[22]
A.R. Lomax and P.C. Calder’ "Prebiotics, immune function, infection and inflammation: a review of the evidence," Br. J. Nutr., vol. 101, pp. 633-658, March 2009.
[23]
S. Madla, P. Methacanon, M. Prasitsil, and K. Kirtikara, "Characterization of biocompatible fungi-derived polymers that induce IL-8 production," Carbohydr. Polym., Vol. 59, pp. 275-280, February 2005.
[24]
T.S. Manning and G.R. Gibson, "Prebiotics," Best Pract. Res. Clin. Gastroentero., vol. 18, pp. 287-298, April 2004.
[25]
W.H. McNeely and K.S. Kang, "Xanthan and other biosynthetic gums," in Industrial Gums, R.L. Whistler, J.N. BeMiller, Eds. New York: Academic, 1973, pp. 473-497.
[26]
P. Methacanon, S. Madla, K. Kirtikara, and M. Prasitsil, "Structural elucidation of bioactive fungi-derived polymers," Carbohydr. Polym., vol. 60, pp. 199-203, July 2008.
[27]
R.P. Oliveira, A.C. Florence, R.C. Silva, P. Perego, A. Converti, L.A. Gioielli, and M.N. Oliveira, "Effect of different prebiotics on the fermentation kinetics, probiotic survival and fatty acids profiles in nonfat symbiotic fermented milk," Int. J. Food Microbiol., vol. 128, pp. 467-472, January 2009.
[28]
X. Pan, T. Wu, L. Zhang, L. Cai, and Z. Song, "Influence of oligosaccharides on the growth and tolerance capacity of lactobacilli to simulated stress environment," Lett. Appl. Microbiol., vol. 48, pp. 362-367, March 2009.
[29]
X.D. Pan, F.Q. Chen, T.X. Wu, H.G. Tang, and Z.Y. Zhao, "Prebiotic oligosaccharides change the concentrations of short-chain fatty acids and the microbial population of mouse bowel," J. Zhejiang Univ. Sci. B., vol. 10, pp. 258-263, April 2009.
[30]
H. Parracho, A.L. McCartney, and G.R. Gibson, "Probiotics and prebiotics in infant nutrition," Proc. Nutr. Soc., vol. 66, pp. 405-411, August 2007.
[31]
Y. Peng, L. Zhang, F. Zeng, and Y. Xu, "Structure and antitumor activity of extracellular polysaccharides from mycelium," Carbohydr. Polym., vol. 54 pp. 297-303, November 2003.
[32]
A. Pompei, L. Cordisco, S. Raimondi, A. Amaretti, U.M. Pagnoni, D. Matteuzzi, and M. Rossi, "In vitro comparison of the prebiotic effects of two inulin-type fructans," Anaerobe, vol. 14, 280-286, November 2008.
[33]
M.B. Roberfroid, "Prebiotics: preferential substrates for specific germs?," Am. J. Clin. Nutr., vol. 73, pp. 406S-409S, February 2001.
[34]
M.B. Roberfroid, "Prebiotics: the concept revisited," J. Nutr., vol. 137, pp. 830S-837S. March 2007.
[35]
N. Salazar, M. Gueimonde, A.M. Hernandez-Barranco, P. Ruas-Madiedo, G. Clara, and L. Reyes-Gavilan, "Exopolysaccharides produced by intestinal Bifidobacterium strains act as fermentable substrates for human intestinal bacteria," Appl. Environ. Microbiol., vol. 74, pp. 4737-4745, August 2008.
[36]
N. Salazar, P. Ruas-Madiedo, S. Kolida, M. Collins, R. Rastall, G. Gibson, and C.G. de Los Reyes-Gavilán, "Exopolysaccharides produced by Bifidobacterium longum IPLA E44 and Bifidobacterium animalis subsp. lactis IPLA R1 modify the composition and metabolic activity of human fecal microbiota in pH-controlled batch cultures," Int. J. Food Microbiol., vol. 135, pp. 260-267, November 2009.
[37]
D.M. Saulnier, J.K. Spinler, G.R. Gibson, and J. Versalovic, "Mechanisms of probiosis and prebiosis: considerations for enhanced functional foods," Curr. Opin. Biotechnol., vol. 20, pp. 135-141, April 2009.
[38]
P. Su, A. Henriksson and H. Mitchell, "Selected prebiotics support the growth of probiotic mono-cultures in vitro," Anaerobe, vol. 13, pp.134-139, June-August 2007.
[39]
K. Swennen, C.M. Courtin, and J.A. Delcour, "Non-digestible oligosaccharides with prebiotic properties," Crit. Rev. Food Sci. Nutr., vol. 46, pp. 459-471, June 2006.
[40]
A. Synytsya, K. Míčkova, A. Synytsya, I. Jablonský, and J. Spěváček, "Glucans from fruit bodies of cultivated mushrooms Pleurotus ostreatus and Pleurotus eryngii: Structure and potential prebiotic activity," Carbohydr. Polym., vol. 76, pp. 548-556, May 2009.
[41]
P. Trevisi, S. De Filippi, L. Minieri, M. Mazzoni, M. Modesto, B. Biavati, and P. Bosi, "Effect of fructo-oligosaccharides and different doses of Bifidobacterium animalis in a weaning diet on bacterial translocation and Toll-like receptor gene expression in pigs," Nutr. Vol. 24, 1023-1029, October 2008.
[42]
L. Vuyst and B. Degeest, "Heteropolysaccharides from lactic acid bacteria," FEMS Microbiol. Rev., vol. 23, pp. 153–177, April 1999.
[43]
C.P. Xu and J.W. Yun, "Influence of aeration on the production and the quality of the exopolysaccharides from Paecilomyces tenuipes C240 in a stirred-tank fermenter," Enz. Microb. Technol., vol. 35, pp. 33-39, July 2004.
[44]
S.K. Yeo and M.T. Liong, "Effect of prebiotics on viability and growth characteristics of probiotics in soymilk," J. Sci. Food Agric., vol. 90, pp. 267–275, January 2010.
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